il 8 gene Search Results


gene exp il8 bt03211906 m1  (Thermo Fisher)
86
Thermo Fisher gene exp il8 bt03211906 m1
Gene Exp Il8 Bt03211906 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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expression vector pcmv3  (Sino Biological)
93
Sino Biological expression vector pcmv3
Expression Vector Pcmv3, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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gene exp il8 ss03392437 m1  (Thermo Fisher)
94
Thermo Fisher gene exp il8 ss03392437 m1
Gene Exp Il8 Ss03392437 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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gene exp il8 oc03397860 m1  (Thermo Fisher)
85
Thermo Fisher gene exp il8 oc03397860 m1
OE reduces inflammation in rabbit disc cells in vitro, increases collagen gene expression in vivo. A – C Rabbit disc cells were pretreated with different concentrations of OE and then stimulated with lipopolysaccharide (LPS) for 16 h. Gene expression of Interleukin-8 <t>(IL-8)</t> and nitric oxide synthase 2 (NOS2) was analyzed. Statistically significant differences between OE-treated and VC (saline) from LPS (P < 0.05) are indicated (*). Conditioned media was collected to quantify interleukin-8 (IL-8) protein. (Lower panel D) Rabbits (n = 8) underwent surgical disc injury and intradiscal treatment with OE or VC. After 3 weeks, intervertebral disc tissues were isolated for RNA analysis of phenotypic disc and inflammatory genes: collagen type I (COL1), collagen type II (COL2), chemokine C–C motif ligand (CCL)2, CCL5 and IL-8 ( D ). Cell culture experiment data were analyzed with One way ANOVA with multiple comparisons and included in the significant difference (*) on graphs. In vivo, rabbit disc gene expression was analyzed with multiple unpaired t-tests. Error bars represent SEM
Gene Exp Il8 Oc03397860 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp il8 oc03397860 m1/product/Thermo Fisher
Average 85 stars, based on 1 article reviews
gene exp il8 oc03397860 m1 - by Bioz Stars, 2026-03
85/100 stars
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gene exp il8 bt03211908 m1  (Thermo Fisher)
85
Thermo Fisher gene exp il8 bt03211908 m1
OE reduces inflammation in rabbit disc cells in vitro, increases collagen gene expression in vivo. A – C Rabbit disc cells were pretreated with different concentrations of OE and then stimulated with lipopolysaccharide (LPS) for 16 h. Gene expression of Interleukin-8 <t>(IL-8)</t> and nitric oxide synthase 2 (NOS2) was analyzed. Statistically significant differences between OE-treated and VC (saline) from LPS (P < 0.05) are indicated (*). Conditioned media was collected to quantify interleukin-8 (IL-8) protein. (Lower panel D) Rabbits (n = 8) underwent surgical disc injury and intradiscal treatment with OE or VC. After 3 weeks, intervertebral disc tissues were isolated for RNA analysis of phenotypic disc and inflammatory genes: collagen type I (COL1), collagen type II (COL2), chemokine C–C motif ligand (CCL)2, CCL5 and IL-8 ( D ). Cell culture experiment data were analyzed with One way ANOVA with multiple comparisons and included in the significant difference (*) on graphs. In vivo, rabbit disc gene expression was analyzed with multiple unpaired t-tests. Error bars represent SEM
Gene Exp Il8 Bt03211908 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp il8 bt03211908 m1/product/Thermo Fisher
Average 85 stars, based on 1 article reviews
gene exp il8 bt03211908 m1 - by Bioz Stars, 2026-03
85/100 stars
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gene exp il8 ec03468860 m1  (Thermo Fisher)
94
Thermo Fisher gene exp il8 ec03468860 m1
Primer and probe source list.
Gene Exp Il8 Ec03468860 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp il8 ec03468860 m1/product/Thermo Fisher
Average 94 stars, based on 1 article reviews
gene exp il8 ec03468860 m1 - by Bioz Stars, 2026-03
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gene exp il8 bt03211907 g1  (Thermo Fisher)
85
Thermo Fisher gene exp il8 bt03211907 g1
The impact of As on S. aureus ClfB expression and MAC-T cells inflammatory response. A describes the experimental design (1-exposure of S. aureus to MAC-T cells; 2-adhesion/infection of MAC-T cells with S. aureus to; 3-As inclusion during infection and inhibition of ClfB; 4-MAC-T cells pro-inflammatory response). B shows the impact of As on IL6, IL1β, <t>IL8</t> and TNFα expression in infected MAC-T cells. C indicates the effect of As on ClfB expression in S. aureus without the presence of MAC-T cells; the expression ClfB in S-aureus co-cocultured with MAC-T cells and As is presented in panel D , E indicates the expression of ClfB in S. aureus MAC-T cells attached or internalised after 6 h of exposure to 0.1, 0.2, 0.5 and 1% As. All experiments were performed in triplicate and the results are represented as means ± SD. Student’s t test was performed to assess significance with the P values being indicated on graphs. The ClfB gene expression is presented as fold change over the infected and As unexposed control. Panel A was created using Biorender
Gene Exp Il8 Bt03211907 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp il8 bt03211907 g1/product/Thermo Fisher
Average 85 stars, based on 1 article reviews
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gene exp il8 mf02789706 g1  (Thermo Fisher)
86
Thermo Fisher gene exp il8 mf02789706 g1
The impact of As on S. aureus ClfB expression and MAC-T cells inflammatory response. A describes the experimental design (1-exposure of S. aureus to MAC-T cells; 2-adhesion/infection of MAC-T cells with S. aureus to; 3-As inclusion during infection and inhibition of ClfB; 4-MAC-T cells pro-inflammatory response). B shows the impact of As on IL6, IL1β, <t>IL8</t> and TNFα expression in infected MAC-T cells. C indicates the effect of As on ClfB expression in S. aureus without the presence of MAC-T cells; the expression ClfB in S-aureus co-cocultured with MAC-T cells and As is presented in panel D , E indicates the expression of ClfB in S. aureus MAC-T cells attached or internalised after 6 h of exposure to 0.1, 0.2, 0.5 and 1% As. All experiments were performed in triplicate and the results are represented as means ± SD. Student’s t test was performed to assess significance with the P values being indicated on graphs. The ClfB gene expression is presented as fold change over the infected and As unexposed control. Panel A was created using Biorender
Gene Exp Il8 Mf02789706 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp il8 mf02789706 g1/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
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gene exp il8 ss03392435 m1  (Thermo Fisher)
86
Thermo Fisher gene exp il8 ss03392435 m1
The impact of As on S. aureus ClfB expression and MAC-T cells inflammatory response. A describes the experimental design (1-exposure of S. aureus to MAC-T cells; 2-adhesion/infection of MAC-T cells with S. aureus to; 3-As inclusion during infection and inhibition of ClfB; 4-MAC-T cells pro-inflammatory response). B shows the impact of As on IL6, IL1β, <t>IL8</t> and TNFα expression in infected MAC-T cells. C indicates the effect of As on ClfB expression in S. aureus without the presence of MAC-T cells; the expression ClfB in S-aureus co-cocultured with MAC-T cells and As is presented in panel D , E indicates the expression of ClfB in S. aureus MAC-T cells attached or internalised after 6 h of exposure to 0.1, 0.2, 0.5 and 1% As. All experiments were performed in triplicate and the results are represented as means ± SD. Student’s t test was performed to assess significance with the P values being indicated on graphs. The ClfB gene expression is presented as fold change over the infected and As unexposed control. Panel A was created using Biorender
Gene Exp Il8 Ss03392435 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp il8 ss03392435 m1/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
gene exp il8 ss03392435 m1 - by Bioz Stars, 2026-03
86/100 stars
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gene exp il8 oc03397858 m1  (Thermo Fisher)
86
Thermo Fisher gene exp il8 oc03397858 m1
The impact of As on S. aureus ClfB expression and MAC-T cells inflammatory response. A describes the experimental design (1-exposure of S. aureus to MAC-T cells; 2-adhesion/infection of MAC-T cells with S. aureus to; 3-As inclusion during infection and inhibition of ClfB; 4-MAC-T cells pro-inflammatory response). B shows the impact of As on IL6, IL1β, <t>IL8</t> and TNFα expression in infected MAC-T cells. C indicates the effect of As on ClfB expression in S. aureus without the presence of MAC-T cells; the expression ClfB in S-aureus co-cocultured with MAC-T cells and As is presented in panel D , E indicates the expression of ClfB in S. aureus MAC-T cells attached or internalised after 6 h of exposure to 0.1, 0.2, 0.5 and 1% As. All experiments were performed in triplicate and the results are represented as means ± SD. Student’s t test was performed to assess significance with the P values being indicated on graphs. The ClfB gene expression is presented as fold change over the infected and As unexposed control. Panel A was created using Biorender
Gene Exp Il8 Oc03397858 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp il8 oc03397858 m1/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
gene exp il8 oc03397858 m1 - by Bioz Stars, 2026-03
86/100 stars
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Image Search Results


OE reduces inflammation in rabbit disc cells in vitro, increases collagen gene expression in vivo. A – C Rabbit disc cells were pretreated with different concentrations of OE and then stimulated with lipopolysaccharide (LPS) for 16 h. Gene expression of Interleukin-8 (IL-8) and nitric oxide synthase 2 (NOS2) was analyzed. Statistically significant differences between OE-treated and VC (saline) from LPS (P < 0.05) are indicated (*). Conditioned media was collected to quantify interleukin-8 (IL-8) protein. (Lower panel D) Rabbits (n = 8) underwent surgical disc injury and intradiscal treatment with OE or VC. After 3 weeks, intervertebral disc tissues were isolated for RNA analysis of phenotypic disc and inflammatory genes: collagen type I (COL1), collagen type II (COL2), chemokine C–C motif ligand (CCL)2, CCL5 and IL-8 ( D ). Cell culture experiment data were analyzed with One way ANOVA with multiple comparisons and included in the significant difference (*) on graphs. In vivo, rabbit disc gene expression was analyzed with multiple unpaired t-tests. Error bars represent SEM

Journal: Journal of Translational Medicine

Article Title: Phenylpropanoid-enriched broccoli seedling extract can reduce inflammatory markers and pain behavior

doi: 10.1186/s12967-023-04777-1

Figure Lengend Snippet: OE reduces inflammation in rabbit disc cells in vitro, increases collagen gene expression in vivo. A – C Rabbit disc cells were pretreated with different concentrations of OE and then stimulated with lipopolysaccharide (LPS) for 16 h. Gene expression of Interleukin-8 (IL-8) and nitric oxide synthase 2 (NOS2) was analyzed. Statistically significant differences between OE-treated and VC (saline) from LPS (P < 0.05) are indicated (*). Conditioned media was collected to quantify interleukin-8 (IL-8) protein. (Lower panel D) Rabbits (n = 8) underwent surgical disc injury and intradiscal treatment with OE or VC. After 3 weeks, intervertebral disc tissues were isolated for RNA analysis of phenotypic disc and inflammatory genes: collagen type I (COL1), collagen type II (COL2), chemokine C–C motif ligand (CCL)2, CCL5 and IL-8 ( D ). Cell culture experiment data were analyzed with One way ANOVA with multiple comparisons and included in the significant difference (*) on graphs. In vivo, rabbit disc gene expression was analyzed with multiple unpaired t-tests. Error bars represent SEM

Article Snippet: Taqman gene expression assays were used for analysis or normalization of the following genes: rabbit collagen type I (COL1), collagen type II (COL2), IL-8, hypoxanthine phosphoribosyltransferase 1 (HPRT1) and 18 S rRNA (Oc03396113_m1, Oc03396134_m1, Oc03397860_m1, Oc03399461_m1, Hs99999901_s1; all from Applied Biosystems, Foster City, CA).

Techniques: In Vitro, Gene Expression, In Vivo, Saline, Isolation, Cell Culture

Primer and probe source list.

Journal: Frontiers in Veterinary Science

Article Title: A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1

doi: 10.3389/fvets.2021.674850

Figure Lengend Snippet: Primer and probe source list.

Article Snippet: IL-8 , TaqMan ® gene expression assay no: Ec03468860_ml (Thermo Fisher).

Techniques: Gene Expression

Cell viability and chemokine mRNA expression following Flu Avert treatment in ERECs. (A) Microscopic analysis of EREC cultures. Mean grades from cells ± SEM of three horses following Flu Avert treatment at different MOIs. Grading of cell viability from 0 to 3 is described in . (B) Cell viability analysis. Mean percent positive cells ± SEM of three horses at each MOI as determined with propidium iodide staining. Black circle is MOI = 0. Black square is MOI = 0.1. Black upright triangle is MOI = 1. Black upside-down triangle is MOI = 5. (C) IL-8 mRNA expression. (D) CXCL9 mRNAs expression. (E) CCL2 mRNA expression. Values are mean log fold change (–ddCq) ± SEM. Black square represents untreated (media) treated ERECs. Black circle represents Flu Avert-treated ERECs. * p ≤ 0.05 and ** p < 0.01, respectively, between the media and Flu Avert treatment groups.

Journal: Frontiers in Veterinary Science

Article Title: A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1

doi: 10.3389/fvets.2021.674850

Figure Lengend Snippet: Cell viability and chemokine mRNA expression following Flu Avert treatment in ERECs. (A) Microscopic analysis of EREC cultures. Mean grades from cells ± SEM of three horses following Flu Avert treatment at different MOIs. Grading of cell viability from 0 to 3 is described in . (B) Cell viability analysis. Mean percent positive cells ± SEM of three horses at each MOI as determined with propidium iodide staining. Black circle is MOI = 0. Black square is MOI = 0.1. Black upright triangle is MOI = 1. Black upside-down triangle is MOI = 5. (C) IL-8 mRNA expression. (D) CXCL9 mRNAs expression. (E) CCL2 mRNA expression. Values are mean log fold change (–ddCq) ± SEM. Black square represents untreated (media) treated ERECs. Black circle represents Flu Avert-treated ERECs. * p ≤ 0.05 and ** p < 0.01, respectively, between the media and Flu Avert treatment groups.

Article Snippet: IL-8 , TaqMan ® gene expression assay no: Ec03468860_ml (Thermo Fisher).

Techniques: Expressing, Staining

Effect of Flu Avert treatment on chemokine mRNA expression in ERECs collected from five horses 24 h following EHV-1 inoculation. (A) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 5 prior to EHV-1 inoculation. (B) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 7 prior to EHV-1 inoculation. (C) CXCL10 mRNA expression in ERECs that were treated with Flu Avert on day 5 prior to EHV-1 inoculation. (D) CXCL10 mRNA expression in ERECs that were treated with Flu Avert on day 7 prior to EHV-1 inoculation. The mean log fold change (–ddCq) is represented by a bar (* p < 0.05).

Journal: Frontiers in Veterinary Science

Article Title: A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1

doi: 10.3389/fvets.2021.674850

Figure Lengend Snippet: Effect of Flu Avert treatment on chemokine mRNA expression in ERECs collected from five horses 24 h following EHV-1 inoculation. (A) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 5 prior to EHV-1 inoculation. (B) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 7 prior to EHV-1 inoculation. (C) CXCL10 mRNA expression in ERECs that were treated with Flu Avert on day 5 prior to EHV-1 inoculation. (D) CXCL10 mRNA expression in ERECs that were treated with Flu Avert on day 7 prior to EHV-1 inoculation. The mean log fold change (–ddCq) is represented by a bar (* p < 0.05).

Article Snippet: IL-8 , TaqMan ® gene expression assay no: Ec03468860_ml (Thermo Fisher).

Techniques: Expressing

Effect of Flu Avert treatment on chemokine mRNA expression in ERECs collected from five horses 48 h following EHV-1 inoculation. (A) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 1 prior to EHV-1 inoculation. (B) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 2 prior to EHV-1 inoculation. (C) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 5 prior to EHV-1 inoculation. (D) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 7 prior to EHV-1 inoculation. (E) CCL2 mRNA expression in ERECs that were treated with Flu Avert on day 1 prior to EHV-1 inoculation. (F) CCL2 mRNA expression in ERECs that were treated with Flu Avert on day 2 prior to EHV-1 inoculation. (G) CCL2 mRNA expression in ERECs that were treated with Flu Avert on day 5 prior to EHV-1 inoculation. (H) CCL2 mRNA expression in ERECs that were treated with Flu Avert on day 7 prior to EHV-1 inoculation. The mean log fold change (–ddCq) is represented by a bar (** p < 0.01 and * p < 0.05).

Journal: Frontiers in Veterinary Science

Article Title: A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1

doi: 10.3389/fvets.2021.674850

Figure Lengend Snippet: Effect of Flu Avert treatment on chemokine mRNA expression in ERECs collected from five horses 48 h following EHV-1 inoculation. (A) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 1 prior to EHV-1 inoculation. (B) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 2 prior to EHV-1 inoculation. (C) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 5 prior to EHV-1 inoculation. (D) IL-8 mRNA expression in ERECs that were treated with Flu Avert on day 7 prior to EHV-1 inoculation. (E) CCL2 mRNA expression in ERECs that were treated with Flu Avert on day 1 prior to EHV-1 inoculation. (F) CCL2 mRNA expression in ERECs that were treated with Flu Avert on day 2 prior to EHV-1 inoculation. (G) CCL2 mRNA expression in ERECs that were treated with Flu Avert on day 5 prior to EHV-1 inoculation. (H) CCL2 mRNA expression in ERECs that were treated with Flu Avert on day 7 prior to EHV-1 inoculation. The mean log fold change (–ddCq) is represented by a bar (** p < 0.01 and * p < 0.05).

Article Snippet: IL-8 , TaqMan ® gene expression assay no: Ec03468860_ml (Thermo Fisher).

Techniques: Expressing

The impact of As on S. aureus ClfB expression and MAC-T cells inflammatory response. A describes the experimental design (1-exposure of S. aureus to MAC-T cells; 2-adhesion/infection of MAC-T cells with S. aureus to; 3-As inclusion during infection and inhibition of ClfB; 4-MAC-T cells pro-inflammatory response). B shows the impact of As on IL6, IL1β, IL8 and TNFα expression in infected MAC-T cells. C indicates the effect of As on ClfB expression in S. aureus without the presence of MAC-T cells; the expression ClfB in S-aureus co-cocultured with MAC-T cells and As is presented in panel D , E indicates the expression of ClfB in S. aureus MAC-T cells attached or internalised after 6 h of exposure to 0.1, 0.2, 0.5 and 1% As. All experiments were performed in triplicate and the results are represented as means ± SD. Student’s t test was performed to assess significance with the P values being indicated on graphs. The ClfB gene expression is presented as fold change over the infected and As unexposed control. Panel A was created using Biorender

Journal: Irish Veterinary Journal

Article Title: The mechanistic role of natural antimicrobials in preventing Staphylococcus aureus invasion of MAC-T cells using an in vitro mastitis model

doi: 10.1186/s13620-024-00265-0

Figure Lengend Snippet: The impact of As on S. aureus ClfB expression and MAC-T cells inflammatory response. A describes the experimental design (1-exposure of S. aureus to MAC-T cells; 2-adhesion/infection of MAC-T cells with S. aureus to; 3-As inclusion during infection and inhibition of ClfB; 4-MAC-T cells pro-inflammatory response). B shows the impact of As on IL6, IL1β, IL8 and TNFα expression in infected MAC-T cells. C indicates the effect of As on ClfB expression in S. aureus without the presence of MAC-T cells; the expression ClfB in S-aureus co-cocultured with MAC-T cells and As is presented in panel D , E indicates the expression of ClfB in S. aureus MAC-T cells attached or internalised after 6 h of exposure to 0.1, 0.2, 0.5 and 1% As. All experiments were performed in triplicate and the results are represented as means ± SD. Student’s t test was performed to assess significance with the P values being indicated on graphs. The ClfB gene expression is presented as fold change over the infected and As unexposed control. Panel A was created using Biorender

Article Snippet: The primers used. cDNA (1 μl) was added to duplicate wells with 7 μl of DNase-free water, 10 μl of Taqman Fast Advanced master mix (Applied Biosystems) and 1 μl of each of 2 Taqman gene expression assays (Applied Biosystems) ( IL-6 NM_173923.2 Bt03211903_m1 FAM 69, IL-8 NM_173925.2 Bt03211907_g1 FAM 105, TNFα NM_173966.3 Bt03259155_g1 VIC 66, IL-1β NM_174093.1 Bt03212744_m1 FAM 69).

Techniques: Expressing, Infection, Inhibition, Gene Expression, Control